Synthesis of SPIO Nanoparticles and the Subsequent Applications in Stem Cell Labeling for Parkinson’s Disease

Abstract Parkinson's disease (PD) is characterized by the progressive loss of dopaminergic neurons in midbrain, and stem cell transplantation method provides a promising strategy for treatment. In these studies, tracking biological behaviors transplanted cells vivo essential basic understanding function evaluation clinical effectiveness. present study, we developed novel ultrasmall superparamagnetic iron oxide nanoparticles coating with polyacrylic acid (PAA) methoxypolyethylene glycol amine (PEG) thermal decomposition two-step modification. The USPIO-PAA/PEG NPs have uniform diameter 10.07 ± 0.55 nm proper absorption peak corresponding ligands, as showed TEM FTIR. MTT that survival incubated remained above 96%. synthesized had good relaxation rate 84.65 s ?1 Mm , indicating they could be efficiently uptake traced MRI. Furthermore, primary human adipose-derived (HADSCs) were characterized, labeled into striatum 6-hydroxydopamine (6-OHDA)-induced PD rat models. MRI up to 3 weeks after surgery; moreover, HADSCs significantly attenuated apomorphine-induced rotations models increased number substania nigra . Overall, development tool tracing technique therapy identifies track therapeutic potential PD.